4992423
| For purification of molecular biology grade from 1-5 ml overnight bacteria culture |
TIANprep Mini Plasmid Kit is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt.
Plasmid DNA purified with TIANprep Mini Plasmid Kit is immediately ready for use. Phenol extraction and ethanol precipitation are not required. High-quality plasmid DNA is eluted in a small volume of Tris Buffer or deionized water. This protocol is designed for purification of plasmid DNA from 1-5ml overnight cultures of E. coli in LB (Luria-Bertani) medium.
Plasmid DNA prepared by TIANprep Mini Plasmid Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of culture cells.
